Plasmid_Backbone

Part:BBa_J99003:Design

Designed by: David Bikard   Group: Mazel Lab   (2009-12-17)

pSUlib ori_p15A; [KmR]


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2242
    Illegal NheI site found at 2066
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2248
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2242
    Illegal BglII site found at 950
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2242
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2242
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2257
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 286
    Illegal AgeI site found at 1655
    Illegal AgeI site found at 1978
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SapI site found at 226
    Illegal SapI site found at 436


Design Notes

Modification of the MCS of pSU38delta by inverse PCR with oligo 5'-GCTTCTAGAGTACTAGTAGCGGCCGCTGCAGATTCCGAAGCCCAACCTTTCATA-3' and 5'-AGTACTCTAGAAGCGGCCGCGAATTCTAACATACGAGCCGGAAGCAT-3'


Source

pSU38delta (Biskri et al., 2005)

References

Biskri, L. et al. Comparative study of class 1 integron and Vibrio cholerae superintegron integrase activities. Journal of bacteriology 187, 1740-50(2005).